EFFECT OF SOME FACTORS ON MICROPROPAGATION OF Andrographis paniculata
DOI:
https://doi.org/10.62985/j.huit_ojs.vol26.no4.671Keywords:
Andrographis paniculata, propagation, in vitro, plant growth regulators.Abstract
Andrographis paniculata has been shown to have various beneficial effects, including antibacterial, antiviral, hepatoprotective, anti-inflammatory, antidiabetic, and anticancer. It is typically grown from seeds, but seed dormancy is a major obstacle in commerce. Although hormonal treatments and hot water seed pretreatment have been applied to overcome dormancy, these methods remain insufficient to meet production demands due to plant-to-plant variability, low rooting rates, and slow seedling growth. This study investigated the effects of different concentrations of plant growth regulators (benzyladenine and kinetin) on shoot regeneration and multiplication of Andrographis paniculata. The nodal explants were sterilized with Javel 25% for 7 minutes for optimal efficiency in creating the initial material source. The MS medium supplemented with 1.0 mg.L-1 benzyladenine resulted in a 100% shoot induction rate. The highest shoot multiplication rate was achieved on MS medium containing 0.5 mg.L-1 benzyladenine combined with 1.0 mg.L-1 kinetin. Half-strength MS medium supplemented with 0.5 mg.L-1 Indole-3-butyric acid was suitable for in vitro rooting of Andrographis paniculata.
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