EFFECTS OF ULTRASOUND- AND MICROWAVE-ASSISTED EXTRACTION ON TERPENOIDS RECOVERY FROM Cyperus esculentus AND EVALUATION OF ANTIBACTERIAL ACTIVITY
DOI:
https://doi.org/10.62985/j.huit_ojs.vol26.no4.666Keywords:
Cyperus esculentus, terpenoids, vi sóng, siêu âm, kháng khuẩn.Abstract
Terpenoids from plants are a source of bioactivities, including antibacterial, antioxidant, anti-inflammatory. This study focused on the recovery of terpenoids from Cyperus esculentus using microwave-assisted extraction (MAE), ultrasound-assisted extraction (UAE) and evaluation of the bioactivity of the extracts. The purpose of these two extraction methods was to compare the efficiency of terpenoids recovery from C. esculentus. In addition, this method demonstrated a significant reduction in energy consumption and processing time and was evaluated as effective for the recovery of bioactive natural organic compounds from plants. For the microwave-assisted extraction method with a material/solvent ratio of 1/20 w/v, a microwave power of 360 W, and a microwave time of 2 min, the terpenoids content was 83.37 ± 0.97 mg/gck. The two main factors investigated in the ultrasound-assisted extraction process were ultrasound power (%) and ultrasound time (min), the extraction efficiency was evaluated through the terpenoids content. The research results showed that, under the condition of 25% ultrasound amplitude and 9 min of ultrasound time, the terpenoids content reached 78.79 ± 1.00 mg/gck. Terpenoids have antibacterial properties against Gram-negative and Gram-positive strains such as S. aureus, B. subtilis, E. coli, and Salmonella.
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