ULTRASOUND-ASSISTED EXTRACTION OF ANTIOXIDANT COMPOUNDS FROM LA GIANG (Aganonerion polymorphum Pierre ex Spire)
DOI:
https://doi.org/10.62985/j.huit_ojs.vol26.no3.434Keywords:
Aganonerion polymorphum, ultrasound-assisted extraction, polyphenols, flavonoids, antioxidant activityAbstract
Aganonerion polymorphum Pierre ex Spire, commonly known as lá giang, is an indigenous medicinal plant that contains various bioactive compounds, notably total polyphenols (TPC) and total flavonoids (TFC), which exhibit potential antioxidant activity. However, research on ultrasound-assisted extraction of bioactive compounds from A. polymorphum, and the influence of factors like drying temperature, extraction solvents, ultrasonic temperature, and duration, remains limited. Therefore, this study was conducted to evaluate the effects of these parameters on the yield of TPC and TFC from A. polymorphum using UAE. The experimental design included the following conditions: drying temperatures of 45; 50; 55; 60; and 65 °C; extraction with a 1:10 (w/v) solvent-to-sample ratio using water and ethanol at concentrations of 50%; 70%; and 90%; and ultrasound treatment at a frequency of 37 kHz, under temperatures of 28 ± 0.5; 40; 50; and 60 °C for durations of 5; 10; 15; and 20 minutes. The analytical parameters included the quantification of TPC (expressed as µg gallic acid equivalents per 100 g dry weight [dw]), TFC (expressed as µg quercetin equivalents per 100 g dw), and antioxidant activity measured by IC₅₀ values. The results indicated that a drying temperature of 55 °C was optimal for preserving bioactive compounds in A. polymorphum. The most effective extraction solvent was 70% ethanol. Maximum extraction efficiency for both TPC and TFC was achieved at 50 °C ultrasonic treatment for 10 minutes, yielding 456.57 ± 2.34 (µgGAE/100 g dw) and 138.21 ± 1.37 (µgQE/100 g dw), respectively. The extract also demonstrated notable antioxidant activity, with an IC₅₀ value of 207.50 ± 3.74 µg/mL.
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