EXTRACTION OF CHITIN FROM Sesarma mederi SHELLS USING CHEMICAL TREATMENT
DOI:
https://doi.org/10.62985/j.huit_ojs.vol26.no3.433Keywords:
Chitin, demineralization, HCl, Sesarma mederi shellsAbstract
The shell of Sesarma mederi, a byproduct of fermented crab (mam ba khia) production, is rich in minerals and proteins, requiring proper processing to obtain high-purity chitin. This study examines the effectiveness of hydrochloric acid (HCl) in the demineralization step of chitin extraction. The results show that optimal mineral removal is achieved using a 5% HCl solution with a material-to-solvent ratio of 1:30 (w/v), at 60°C for 3 hours. After demineralization, the sample underwent NaOH treatment to remove residual proteins, yielding chitin with a mineral content below 1.0% and a purity of approximately 97.66%. Structural analysis using Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and scanning electron microscopy (SEM) confirmed the efficient removal of minerals and proteins. The extracted chitin was identified as predominantly α-chitin. This study highlights the potential of conventional chemical processing for chitin recovery from Sesarma mederi shells, offering a sustainable approach to utilizing seafood byproducts and expanding their applications in industrial and biotechnological fields.
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