DEEP EUTECTIC SOLVENT EXTRACTION FOR ASTAXANTHIN FROM GIANT TIGER PRAWN (Penaeus monodon) SHRIMP SHELLS
DOI:
https://doi.org/10.62985/j.huit_ojs.vol26.no3.431Keywords:
Astaxanthin, antioxidant activity, deep eutectic solvent, extraction, giant tiger prawn shrimp shellsAbstract
Astaxanthin, a powerful antioxidant compound commonly used in the food and pharmaceutical industries, was the main focus of this study. The research aimed to evaluate a new method that combines deep eutectic solvent and citric acid for extracting astaxanthin from shrimp waste. The study compared the astaxanthin levels and antioxidant effects of this approach with the traditional ethanol extraction method. The results showed that the highest astaxanthin content (0.311±0.004 mg/g) was achieved by using a mix of choline chloride – glycerol (ChCl-Gl) at a 1:2 molar ratio with an additional 5% citric acid (CG2-5%), extracted at 40 ºC for 3 hours. This led to astaxanthin levels 1.3 times higher than those obtained through ethanol extraction. Additionally, the CG2-5% treatment involving choline chloride – glycerol (ChCl-Gl) and 5% citric acid resulted in cell wall deformation, facilitating a more efficient extraction of astaxanthin, as shown by SEM results. The astaxanthin extracts obtained with CG2-5% demonstrated superior antioxidant properties compared to the ethanol-extracted sample, highlighting the environmentally friendly aspect of the CG2-5% solvent for recovering astaxanthin from shrimp waste.
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